Its cathedral episcopal see is Catedral Santa Florentina, dedicated to Saint Florentina, in Campana and it also has a co-cathedral: Cocatedral Natividad del Señor, dedicated to the Nativity of Our Lord, in Belén de Escobar, also in Buenos Aires Province.
It was created by Pope Paul VI on 21 April 1976, by the Papal Bulla "Qui consilio divino" as Diocese of Zárate–Campana / Zaraten(sis)–Campanen(sis) (Latin adjective), on territories split off from the Diocese of San Isidro and from the Diocese of San Nicolás de los Arroyos. The first bishop, Claretian Mgr. Alfredo Mario Espósito Castro, chosen by Paul VI on 21 April 1976, received his episcopal consecration and took possession of the see on 4 July 1976.
As per 2014, it pastorally served 660,000 Catholics (90.7% of 728,000 total) on 5,924 km² in 33 parishes and 89 missions with 88 priests (53 diocesan, 35 religious), 13 deacons, 178 lay religious (88 brothers, 90 sisters) and 27 seminarians.
It includes the cities of Campana (credited in its title), (Belén de) Escobar, Zarate, Baradero, Pilar, Exaltación de la Cruz and San Antonio de Areco, all in the northern part of Greater Buenos Aires.