The Q25 and Q34 were originally operated by Queens-Nassau Transit Lines, Queens Transit Corporation, and Queens Surface Corporation from the 1930s to 2005; they are now operated by MTA Regional Bus Operations under the MTA Bus Company brand.
The Q25 begins at Poppenhusen Avenue and 119th Street in College Point. The bus then travels via 127th Street, Ulmer Place, and Linden Place, before it meets up with the Q34 at 32nd Avenue. It then moves onto Northern Boulevard, and then onto Main Street in Downtown Flushing (also known as Flushing Chinatown). Here is the Flushing–Main Street terminal, where several bus lines, the IRT Flushing Line subway, and the LIRR Port Washington Branch interchange. It then moves onto Kissena Boulevard, running the entire distance of the street between Main Street and Parsons Boulevard, and then turns via Parsons. The routes proceed south to Jamaica Avenue, then west to Sutphin Boulevard, terminating at Supthin Boulevard and 94th Avenue underneath the Jamaica terminal for the LIRR and AirTrain JFK. This terminal is shared with the parallel Q65 route, also a former Queens Surface route. Between the Whitestone Expressway and Jamaica, the Q25 employs limited-stop service, making intermittent stops primarily at major intersections and points of interest. Local stops are served by the regular Q25 and the Q34.
The only difference in the routes is north of Linden Place, where the Q34 diverges. The Q34 starts at 149th Street and Willets Point Boulevard in Whitestone, and then turns via Union Avenue. The Q34 then turns via local streets until it meets up with the Q25 at Linden Place. The Q34 does not operate during late nights or weekends.
The average daily ridership for the Q25 on weekdays in 2014 was 19,567, the ridership on Saturday was 13,359 and the ridership on Sunday was 10,225. The average daily ridership for the Q34 in 2014 was 7,218.
Q25 service began in 1928, under the operation of the Flushing Heights Bus Company. On May 25, 1933, Queens–Nassau Transit received a one-year franchise for route "Q-34" from Flushing to College Point. The route began service in April 1933.