Born on 15 November 1909 at the Palace of Villamejor, Princess Dolores of Bourbon-Two Sicilies was the second child of Prince Carlos of Bourbon-Two Sicilies and his second wife Princess Louise of Orléans. She was christened Maria de los Dolores Victoria Felipa Luisa Mercedes. Princess Dolores, nicknamed Dola among his relatives, was closely related to the Spanish royal family. Her father, Prince Carlos of Bourbon-Two Sicilies, had renounced his right to the throne of Two Sicilies becoming a Spanish citizen when he married his first wife, Mercedes, Princess of Asturias, the eldest sister of King Alphonso XIII of Spain. Dolores’s mother, Princess Louise of Orléans was a first cousin once removed of the Spanish King. As a result, Dolores and her sibling grew up in close proximity to the Spanish royal family. Her cousins, the children of King Alphonso XIII and Queen Victoria Eugenie were the same age as Dolores and her younger siblings. The family lived at the Palace of Villamejor in Madrid, vacations were spent near Seville in the Palace of Villamanrique, property of her maternal grandmother, Isabelle Countess of Paris.
Princess Dolores studied with her sisters María and Esperanza in a school of Irish nuns in Madrid. Her drawing teacher was the famous painter Manuel González Santos. Dolores was twelve years old when she moved with her family to Seville when her father was appointed Military Captain General of Andalusia. The Princess and her sisters continued their studies as boarders at the school of Irish nuns in Castilleja de la Cueva in Seville. Vacations were usually spent in France with their maternal relatives. The family was very popular in Seville where they lived until April 1931 when the Second Spanish Republic forced them to exile. They moved to Cannes and later to Paris.
In Paris, Princess Dolores met a wealthy Polish aristocrat Prince Augustyn Józef Czartoryski, 13th Prince Czartoryski, Duke of Klewan and Zuków, son of Prince Adam Ludwik Czartoryski and his wife Countess Maria Ludwika Krasińska. They were married on 12 August 1937 in Ouchy, Switzerland. The couple settled in Kraków, Poland where Dolores’s husband took over the running of the Family Museum. Their lives were disrupted with the Second World War. In September 1939 with the Invasion of Poland bombs fell on Kraków, Prince Augustyn and Princess Dolores, who was pregnant, decided to leave the country and move to Spain. In their escape, they were picked up by the Gestapo and put under arrest. It was through the intervention of the Spanish ambassador in Berlin that they were released.
After reaching Paris, Princess Dolores and her husband moved permanently to Spain. They settled in Seville where Princess Dolores gave birth to a son: Prince Adam Karol Czartoryski (born 2 January 1940). Her husband, meanwhile was very active in the Polish resistance. In 1943 the couple bought a rural property in Dos Hermanas which they called it Garden of the Princess, the farm was self-sufficient for the subsistence of the family. The couple had a second son also born in Seville, Prince Ludwik Piotr Czartoryski (13 March 1945 - 3 May 1946), but the child died at only thirteen months.
At the end of the war, the properties of the Czartoryski family were nationalized by the Polish government and the family decided not to return to Kraków, for fear of the Soviet occupation. Prince Augustyn was in poor health, the troubles and tribulation of the war took a toll on his life and he died on 1 July 1946, aged thirty nine. The deaths of both her youngest son and her husband only two months apart in 1946 deeply affected the Princess, who was left a widow with a six-year-old child.