Soon the family moved to industrial city Makeevka located in eastern Ukraine within the Donetsk Province, 25 km (16 mi) from the Donetsk city. Here, Trufanov spent his childhood and teenage years. This time impressions influenced in the future on the formation of the young artist and choose the theme for his main paintings.
In 1937–1940, Trufanov studied at the Odessa Art School, which ended only after World War II in 1945. In 1941-1944, Trufanov took part in the Great Patriotic War. He was wounded and received military awards.
In 1945, Trufanov joined the painting department of the Leningrad Institute of Painting, Sculpture and Architecture named after Ilya Repin. He studied of Boris Fogel, Leonid Ovsannikov, Alexander Zaytsev.
In 1951, Trufanov graduated from Ilya Repin Institute in Boris Ioganson personal Art Studio. His graduation work was a historical painting named "In the headquarters of Kovpak", dedicated to partisan movement in the years of the Great Patriotic War.