Lodowicke Muggleton was born at a house called Walnut Tree Yard on Bishopsgate Street (now Bishopsgate) in the City of London.
His father, John, was a farrier and a post office contractor. Lodowicke was the youngest of three children when his mother, Mary, died in 1612. On his father's remarriage, Lodowicke was put out to nurse in the country, the common practice in such family restructuring at the time. In 1624 he returned to Walnut Tree Yard as an apprentice to a tailor, John Quick. Quick seems to have been well-connected, making ceremonial gowns for Liverymen and Common Councilmen. Muggleton describes him as "a quiet peaceable man, not cruel to servants, which liked me very well". In 1625 Muggleton contracted the plague but, he says, "it was not extreme tedious to me. I recovered quickly, and hath not had half a day's sickness since."
As his apprenticeship drew to a close he began to disdain tailoring as poorly paid. He was offered a stake in a pawnbroker's business by a Mrs Richardson if he would marry her daughter which he seemed keen to do. But he became worried that usury would damn his soul so he remained unmarried, working as a tailor for William Reeve who was John Reeve's elder brother and, at that time, a staunch Puritan. Yet his soul was still troubled "for fear God had made me a reprobate before I was born, because He did not answer my prayers."
His first marriage, 1635, was to Sarah and they had two surviving daughters, Sarah and Elizabeth. After his wife's death he married again but both wife and the children that she bore soon died. Muggleton fell away from the Puritan faith, "for all the zeal we formerly had was quite worn out," and this cost his business dearly in terms of lost customers from that congregation.
It may be possible to recognise some subsequent Muggletonian beliefs as being solutions to the perplexities he felt whilst still a Puritan. Then again, the episodes he chooses to tell in his autobiography may be selected with such a didactic purpose in mind. The idea that conscience is God's watchman within every person, that the conflict between two natures is at work within everyone, and the need to banish the fear of being prey to external spirits all seem to stem from personal exigencies of this period in his life.