On March 14, 2011, Universal announced that the attraction would begin operating on a seasonal schedule starting on April 1, 2011, and would operate for limited amounts of time throughout the spring and summer seasons before it closed completely on August 18, 2011. Jimmy Neutron's Nicktoon Blast was replaced by Despicable Me Minion Mayhem on July 2, 2012.
In spring 2003, Universal Studios Florida released this official synopsis:
After a Universal Studios crew member directs guests into a briefing room, Jimmy Neutron welcomes the guests and, with help from Carl, unveils his newest invention: the Mark IV rocket. Shortly afterwards, Ooblar breaks into the lab so he can return Carl's teddy bear. Then, he claims the Mark IV for his own, flying off through Nicktoons Studios. Quickly, Jimmy announces that he and Carl will follow Ooblar in their Mark II rocket, while the guests will follow in the original (and slightly unpredictable) Mark I rockets. He assures everyone that he will safely pilot the guests by remote, right before it shorts circuits and causes a black-out. The pre-show ends with the lab doors opening and a crew member ushering the guests into the rockets and delivering safety instructions.
Carl nervously counts down from 10 for the launch, but Jimmy interrupts him by starting the rockets and leaving the lab. They make chase for Ooblar through the sound stages, who leads them first through the Rugrats home, with the Mark I rockets almost capturing Angelica by accident. However, Goddard pulls her out of the way just in time using his robo-claw. They leave the soundstage and make way for Fairy World, where Cosmo offers Carl his wand as assistance. The dumbstruck Carl accidentally poofs the rockets (and their passengers) to the Yolkian planet, sending the rockets crashing through the planet's capital city. The chase ends with Jimmy, Carl, and the guests trapped in King Goobot's throne room, where they are confronted by Poultra. They barely escape a fried Ooblar when the engines fail, so Carl, using Cosmo's wand, poofs everyone (Yolkians excluded) back to Earth, where they plummet into Bikini Bottom. SpongeBob, mistaking the Mark II for a jellyfish, ensnares Jimmy in his jellyfishing net, blinding the pilot and sending everyone crashing through downtown, ending with the Mark II and Mark I rockets going through a drain pipe leading back to Jimmy's lab. Just when everyone thinks they're safe, King Goobot barges in, trapping Jimmy and using the pilot remote to make the ride vehicles do the chicken dance. Carl, realizing that he still has Cosmo's wand, sends out a laser blast that flies over the audience and cracks the king. The ride ends with Carl poofing up a bunch of llamas and Jimmy wishing everyone farewell.
The facility which used to house The Funtastic World of Hanna-Barbera is used for the same purpose as its predecessor. Enough changes were made to re-invent the ride as Jimmy Neutron's Nicktoon Blast, such as paint and a new movie, but it is essentially the same.