Rachel Ames originated the role of Audrey in 1964 in what was originally a thirteen-week stint with an option for an additional thirteen weeks. The character's popularity prompted the series to keep the character on canvas. Ames took an extended leave in 1970 when she had to be put on bedrest during her pregnancy. Maura McGiveney played Audrey temporarily while Ames was gone.
Ames also crossed over to General Hospital's spinoff series Port Charles as Audrey in 1997. In 2002, Ames was dropped to recurring status, and ultimately retired from the series in October 2007. Ames briefly reprised the role for a couple of episodes in October 2009 and reprised the role once again in April 2013 for the series' 50th anniversary.
Audrey March arrives at General Hospital in 1964 to visit her sister, Lucille March (Lucille Wall). She is a registered nurse, who had forsaken the profession for that of an airline stewardess. After noticing Dr. Steve Hardy (John Beradino), the Chief of Internal Medicine at General Hospital, Audrey stays in town and becomes a private nurse. Eventually, Steve and Audrey get engaged, but Audrey breaks it off. She accepts a proposal from one of her clients, Randy Washborn. Audrey is diagnosed with lymphoma, and when Randy deserts her she gets back together with Steve. They marry in 1965 after she is recovered. Audrey later works on a book with Dr. Phil Brewer, but the experience ends badly when Phil tries to seduce her. Audrey becomes concerned when she does not conceive, and believing Steve is sterile, Audrey has herself artificially inseminated without Steve's knowledge and becomes pregnant. Audrey is in a car accident and miscarries; she is devastated and separates from Steve. She goes to Vietnam to help war orphans.
When Audrey returns from Vietnam, she continues to push Steve away and after their divorce is final, she marries Tom Baldwin in an effort to prove that she is over Steve. Audrey cannot bring herself to sleep with Tom, and he ends up raping her. After becoming pregnant with Tom's child, Audrey files for divorce and leaves town, determined to protect her unborn child from her violent husband. She returns in 1971, and tells Lucille her son had died at birth. Audrey reconnects with Steve. It is revealed she is lying about her son, whom she named Steve and is in hiding. She plans to remarry Steve, and then convince him to adopt a baby which would be her son. However, Mrs. Peggy Nelson, the woman taking care of the baby, realizes the plan and blackmails Audrey. Peggy Nelson is murdered and Audrey is accused of the crime. Thanks to Steve, Audrey is acquitted. Tom reclaims baby Steve, who is renamed Tommy, forcing Audrey to resume their marriage. Tom kidnaps baby Tommy and flees to Mexico. Tom is later presumed dead and Tommy is returned to Audrey. During the kidnapping ordeal, Tommy had become ill with a heart ailment. Jim Hobart performs surgery and Tommy recovers. Audrey feels gratitude towards Jim and marries him after he injuries his hands and fears he cannot operate again. Audrey faces heartache as she still loves Steve and Jim turns to alcohol to deal with his injury. In 1976, Jim leaves Audrey for a younger woman. Audrey attempts suicide and Steve saves her. Steve proposes to Audrey and she accepts. Steve falls down a flight of stairs and Audrey helps him recuperate. They remarry, and Tom Baldwin returns alive, invalidating their marriage. Tom tries to reclaim Audrey and his son, but eventually gives up and leaves town. Steve and Audrey marry again in 1977 and Steve officially adopts Tommy, renamed Tom Hardy
Meanwhile, Audrey had become head of student nurses at General Hospital, and as a result becomes a mentor to nurses such as Bobbie Spencer. Steve and Audrey become involved in various dramas involving the children of Steve's old friend Lars Webber, Terri, Rick and Jeff. Jeff ends up shooting himself, and when he is in the hospital, Terri tells Steve about a letter her mother, Helene, had told her about on her deathbed. Steve finds the letter and learns Jeff is actually his biological son (Steve had relationship with Helene during a time when she was separated from Lars). Steve tells Audrey and they agree to keep the truth a secret. In the winter of 1979, Port Charles is hit by an epidemic of Lassa Fever. General Hospital is placed under quarantine, and when Steve begins to fall victim to the disease, Audrey tells Jeff the truth. Steve recovers and both Rick and Jeff are angry at Steve and Audrey. It takes months for everyone to reconcile. Steve and Audrey are happy to learn that Jeff's son Steven Lars Webber is still alive. Jeff soon leaves town with his son to make a fresh start.
Some years later, Tom leaves to attend college and returns in 1987. When Tom marries an African-American doctor, Simone Ravelle, Audrey fears her son's interracial marriage would not be accepted. In time, Audrey and Simone grow close. Simone confides in Audrey when she gets pregnant during an affair with Harrison and does not know who the father is. However, Audrey is relieved when Tom is confirmed to be the little Tommy's father.