There were 16 NOCs competing at this event. These 16 NOCs were selected by the average of the two best marks at the qualifying period. Finland and Cuba were qualified but withdrew and were replaced by Thailand and Nigeria.
In 2016, the victorious Russian relay team was disqualified for doping, as Yuliya Chermoshanskaya had her samples reanalyzed and tested positive for two prohibited substances.
Prior to this competition, the existing world and Olympic records were as follows.
No new world or Olympic records were set for this event.
All times shown are in seconds.